Assessing the diagnostic potential of 16SrRNA gene for neonatal sepsis: A tertiary care hospital study in South India.

ABSTRACT:

Background

The need of the hour is to incorporate a rapid assay that could efficiently detect neonatal sepsis. We evaluated the diagnostic utility of 16SrRNA broad-range polymerase chain reaction (PCR) in neonatal sepsis.

Methods

The demographic and clinical details of 100 neonates clinically suspected to have sepsis were collected adopting pretested clinical proforma, followed by baseline laboratory investigations, including blood culture, complete blood counts, and C-reactive protein (CRP). Around, 0.2-0.3 ml of the EDTA blood was subjected to enrichment followed by DNA isolation using the modified spin column method. Based on the blood culture report, neonates were further divided into the suspected sepsis group (n = 50) and the confirmed sepsis group (n = 50). We perform

SUBMITTER: Yadav AK 

PROVIDER: S-EPMC11842920 | biostudies-literature | 2024 Nov-Dec

REPOSITORIES: biostudies-literature