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Ratiometric Imaging for Quantification of Elevated Ca²⁺ in Neurons Using Synthetic Low-Affinity Fluorescent Probe.

ABSTRACT: The availability of various calcium ion (Ca²⁺) fluorescent probes has contributed to revealing physiological events related to intracellular Ca²⁺. However, conventional probes face challenges for quantitatively and selectively visualizing high Ca²⁺ concentrations in cells induced by any stimuli, including biomolecules or electrical signal that disrupt Ca²⁺ homeostasis. In this report, we designed and synthesized a low-affinity ratiometric Ca²⁺ probe, KLCA-Fura, utilizing o-aminophenol-N,N-diacetate-O-methylene-methylphosphinate (APDAP) as a ligand, for which we recently demonstrated the suitability as a new low-affinity ligand for Ca²⁺. KLCA-Fura showed a blue shift in excitation wavelength

SUBMITTER: Kuronuma Y

PROVIDER: S-EPMC11843598 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature

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Dataset Information

Ratiometric Imaging for Quantification of Elevated Ca²⁺ in Neurons Using Synthetic Low-Affinity Fluorescent Probe.

OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Ratiometric Imaging for Quantification of Elevated Ca2+ in Neurons Using Synthetic Low-Affinity Fluorescent Probe.

OmicsDI is part of the ELIXIR infrastructure

Tweets

Ratiometric Imaging for Quantification of Elevated Ca²⁺ in Neurons Using Synthetic Low-Affinity Fluorescent Probe.