Production of HSVd- and PPV-free apricot cultivars by in vitro thermotherapy followed by meristem culture.

ABSTRACT:

Background

The production of virus-free apricots (Prunus armeniaca L.) is essential for controlling viral diseases, exchanging breeding materials without the risk of spreading new diseases, and preserving plant germplasm. Plum pox virus (PPV) is the most devastating disease of the Prunus genus and Hop stunt viroid (HSVd) is prevalent in most apricot-growing regions. It was evaluated whether thermotherapy, etiolation, or a combination of both followed by meristem culture could effectively eliminate PPV and HSVd from 'Canino' and 'Mirlo Rojo' apricot cultivars in vitro.

Results

In the thermotherapy treatments, shoots were exposed to 38ºC and 32ºC, alternating every four hours, for 30, 35, 40, and 45 days. Before this, shoots were acclimated to heat for one day at 28ºC and two

SUBMITTER: Perez-Caselles C 

PROVIDER: S-EPMC11843746 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature