CatGRANULE 2.0: accurate predictions of liquid-liquid phase separating proteins at single amino acid resolution.

ABSTRACT: Liquid-liquid phase separation (LLPS) enables the formation of membraneless organelles, essential for cellular organization and implicated in diseases. We introduce catGRANULE 2.0 ROBOT, an algorithm integrating physicochemical properties and AlphaFold-derived structural features to predict LLPS at single-amino-acid resolution. The method achieves high performance and reliably evaluates mutation effects on LLPS propensity, providing detailed predictions of how specific mutations enhance or inhibit phase separation. Supported by experimental validations, including microscopy data, it predicts LLPS across diverse organisms and cellular compartments, offering valuable insights into LLPS mechanisms and mutational impacts. The tool is freely available at https://tools.tartaglialab.com/catgranul

SUBMITTER: Monti M 

PROVIDER: S-EPMC11843755 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature