The complex I subunit B22 contains a LYR domain that is crucial for an interaction with the mitochondrial acyl carrier protein SDAP1.

ABSTRACT: Mitochondrial complex I (CI), a large multi-subunit respiratory complex contains two LYR (leucine/tyrosine/arginine) domain-containing subunits, B14 (NDUA6/LYRM6) and B22 (NDUB9/LYRM3). Mitochondrial LYR (LYRM) proteins are soluble matrix-located proteins that have been implicated in diverse functions such as iron-sulphur cluster insertion, OXPHOS complex assembly, and mitoribosome biogenesis. B14 and B22 are unique to other LYRM proteins in that they are integral components of CI. To explore the function of B22, we examined T-DNA insertional knockout and knockdown lines, which displayed a mild growth defect linked to reduced CI activity and abundance. Notably, this defect could not be rescued by complementation with a B22 variant that contained a mutated LYR domain, indicating the domain'

SUBMITTER: Saha S 

PROVIDER: S-EPMC11843852 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature