Potential of mir-299-5p to modulate LPS-induced inflammation and osteogenic differentiation of periodontal stem cells by targeting PUM2.

ABSTRACT:

Background

Periodontitis is a prevalent oral disease that significantly impacts human quality of life. This study aimed at the influence of microRNA-299-5p (miR-299-5p) on regulating lipopolysaccharide (LPS)-induced osteogenic differentiation of periodontal ligament stem cells (PDLSCs).

Methods

The 105 periodontitis patients and 102 healthy periodontal volunteers (HC) were recruited with their clinical baseline data. miR-299-5p expression in saliva was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). To identify the target relationship between miR-299-5p and pumilio RNA-binding family member 2 (PUM2), the dual luciferase reporter gene was explored. PDLSCs were treated with LPS and subjected to osteogenic differentiation induction. The secre

SUBMITTER: Zhang QH 

PROVIDER: S-EPMC11843968 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature