Ferroptosis-related genes in preeclampsia: integrative bioinformatics analysis, experimental validation and drug prediction.

ABSTRACT:

Introduction

Preeclampsia (PE) is a severe pregnancy complication with limited early diagnostic and therapeutic options. Ferroptosis, an iron-dependent cell death pathway, has emerged as a potential mechanism in PE pathogenesis. This study investigated ferroptosis-related genes (FRGs) in PE to identify diagnostic biomarkers and therapeutic targets.

Methods

Differentially expressed genes were identified from GEO databases and intersected with FRGs. Hub genes were selected using RandomForest and LASSO algorithms. Their diagnostic potential was evaluated through ROC analysis. Regulatory networks were constructed using transcription factors, microRNAs and potential drug targets. Hub gene expression was validated through immunohistochemistry, Western blot, and RT-qPCR in placenta

SUBMITTER: He L 

PROVIDER: S-EPMC11844108 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature