Up-regulation of m⁶A writer METTL14 inhibits tumorigenesis by suppressing glycolysis in colorectal cancer.

ABSTRACT:

Background

Colorectal cancer (CRC) is a common malignant tumor. N⁶-Methyladenosine (m⁶A) modification plays an important role in the regulation of glycolysis in tumor cells and may be a potential target for tumor therapy.

Methods

The role of METTL14, an m⁶A writer, in CRC was investigated through functional assays including cell viability, colony formation, and glycolysis-related measurements (glucose uptake, lactate production, extracellular acidification rate (ECAR) and oxygen consumption rate (OCR)). The target gene regulated by METTL14 in an m⁶A-dependent manner was identified using molecular biology techniques. In addition, CRC cells overexpressing METTL14 were subcutaneously injected into mice to verify the regulatory effect

SUBMITTER: Li L 

PROVIDER: S-EPMC11844156 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature