Depth and Strain-Dependent Structural Responses of Mouse Lens Fiber Cells During Whole Lens Shape Changes.

ABSTRACT:

Purpose

To study the relationship between whole lens shape changes and fiber cell responses to externally applied loads.

Methods

Freshly dissected mouse lenses were compressed by applying glass coverslips to the lens anterior, followed by fixation to preserve lens shape, and preparation for scanning electron microscopy (SEM). SEM images were collected from the outer cortex to the nucleus, and fiber cell end-to-end curvature and membrane paddle dimensions were measured using ImageJ.

Results

At 23% and 29% axial strain, cortical fiber bundle curvature increased significantly compared to control uncompressed lenses, whereas nuclear fiber bundle curvature was unaffected. Outer cortical fiber cell membrane paddles and protrusions were dramatically distorted in a radial dir

SUBMITTER: Cheheltani S 

PROVIDER: S-EPMC11844228 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature