Melatonin Alleviates Age-Related Lacrimal Gland Dysfunction Via SIRT-1/NLRP3 Pathway.

ABSTRACT:

Purpose

As a consequence of the natural aging process, the lacrimal glands may become dysfunctional. The present study aimed to investigate the potential role of melatonin (MLT) in the alleviation of age-related lacrimal gland dysfunction and to elucidate the underlying mechanism.

Methods

In this study, lacrimal glands of 2-month-old, 18-month-old, and MLT intraperitoneally injected 18-month-old mice were obtained for immunofluorescence, immunohistochemistry experiments, and Western blotting to detect inflammatory factors and AQP5 expression, and for electron microscopy to detect mitochondrial structure and dense granules. Lacrimal glands from 18-month-old mice were taken for cell culture, and PCR and Western blotting were performed to detect the signaling pathways in which

SUBMITTER: Wang C 

PROVIDER: S-EPMC11844230 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature