Estimating Gene Conversion Tract Length and Rate From PacBio HiFi Data.

ABSTRACT: Gene conversions are broadly defined as the transfer of genetic material from a "donor" to an "acceptor" sequence and can happen both in meiosis and mitosis. They are a subset of noncrossover (NCO) events and, like crossover (CO) events, gene conversion can generate new combinations of alleles and counteract mutation load by reverting germline mutations through GC-biased gene conversion. Estimating gene conversion rate and the distribution of gene conversion tract lengths remains challenging. We present a new method for estimating tract length, rate, and detection probability of NCO events directly in HiFi PacBio long read data. The method can be used to make inference from sequencing of gametes from a single individual. The method is unbiased even under low single nucleotide variant (SNV)

SUBMITTER: Charmouh AP 

PROVIDER: S-EPMC11844249 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature