Spatially distinct cellular and molecular landscapes define prognosis in triple negative breast cancer.

ABSTRACT:

Background-

Triple-negative breast cancer is a prevalent breast cancer subtype with the lowest 5-year survival. Several factors contribute to its treatment response, but the inherent molecular and cellular tumor heterogeneity are increasingly acknowledged as crucial determinants.

Methods-

Spatial transcriptomic profiling was performed on FFPE tissues from a retrospective, treatment-naive group of women with differential prognoses (17 with >15 years survival- good prognosis (GPx) and 15 with <3 years survival-poor prognosis (PPx)) using GeoMX^® Digital Spatial Profiler. Regions of interest were segmented on pan-cytokeratin and analyzed for tumor and stromal components, probed using GeoMx human whole transcriptome atlas (WTA) panel. Data quality control, normalizatio

SUBMITTER: Mukund K 

PROVIDER: S-EPMC11844391 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature