Molecularly-guided spatial proteomics captures single-cell identity and heterogeneity of the nervous system.

ABSTRACT: Single-cell proteomics is an emerging field with significant potential to characterize heterogeneity within biological tissues. It offers complementary insights to single-cell transcriptomics by revealing unbiased proteomic changes downstream of the transcriptome. Recent advancements have focused on enhancing proteome coverage and depth, mostly in cultured cell lines, and a few recent studies have explored the potential of analyzing tissue micro-samples but were limited to homogenous peripheral tissues. In this current work, we utilize the power of spatial single cell-proteomics through immunostaining-guided laser capture microdissection (LCM) coupled with LC-MS to investigate the heterogenous central nervous system. We used this method to compare neuronal populations from cortex and subst

SUBMITTER: Dutta S 

PROVIDER: S-EPMC11844393 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature