TRPV4 Dominates High Shear-Induced Initial Traction Response and Long-Term Relaxation Over Piezo1.

ABSTRACT: Modulation of endothelial traction is critical for the responses of endothelial cells to fluid shear stress (FSS), which has profound implications for vascular health and atherosclerosis. Previously, we demonstrated that under high FSS, endothelial cells rapidly increase traction forces, followed by relaxation, with traction aligning in the flow direction. In contrast, low shear preconditioning induces a modest short-term increase in traction (<30 min), followed by a secondary long-term (>14 hr) rise, with traction/cells aligning perpendicular to the flow. The upstream mechanosensors driving these responses, however, remain unknown. Here, we sought the roles of Piezo1 and TRPV4 ion channels in shear-induced traction modulation. We report that HUVECs with Piezo1 silencing reduced the initia

SUBMITTER: Chandurkar MK 

PROVIDER: S-EPMC11844455 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature