An <i>ex vivo</i> and <i>in vitro</i> investigation of extracellular vesicle interactions with B cells of <i>Macaca nemestrina</i> and humans.

ABSTRACT: Extracellular vesicles may modify recipient cell behavior through multiple mechanisms, including interacting with the cell surface or internal membrane components and delivering luminal cargo to the cytoplasm. Here, we use a previously established ex vivo approach to investigate the cellular fate of EVs spiked into whole blood samples from nonhuman primate (NHP) and human donors and contrast these findings with results from in vitro assays. We report that EVs are internalized by NHP and human B cells while also associating to some degree with other PBMCs. EVs exhibit greater association with B cells in ex vivo whole blood compared to isolated B cells, suggesting that blood components may promote EV interactions or that cell isolation factors may inhibit this associatio

SUBMITTER: Pachane BC 

PROVIDER: S-EPMC11844526 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature