Modeling cerebral development <i>in vitro</i> with L-<i>MYC</i>-immortalized human neural stem cell-derived organoids.

ABSTRACT: A promising advance for ex vivo studies of human brain development and formulation of therapeutic strategies has been the adoption of brain organoids that, to a greater extent than monolayer or spheroid cultures, recapitulate to varying extents the patterns of tissue development and cell differentiation of human brain. Previously, such studies been hampered by limited access to relevant human tissue, inadequate human in vitro models, and the necessity of using rodent models that imperfectly reproduce human brain physiology. Here we present a novel organoid-based research platform utilizing L-MYC-immortalized human fetal neural stem cells (LMNSC01) grown in a physiological 4% oxygen environment. We visualized developmental processes in LMNSC01 brain organoids for over 1

SUBMITTER: Ojeda AV 

PROVIDER: S-EPMC11844543 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature