Scattering Correction through Fourier-Domain Intensity Coupling in Two-Photon Microscopy (2P-FOCUS).

ABSTRACT: Light penetration depth in biological tissue is limited by tissue scattering. Correcting scattering becomes particularly challenging in scenarios with limited photon availability and when access to the transmission side of the scattering tissue is not possible. Here, we introduce a new two-photon microscopy system with Fourier-domain intensity coupling for scattering correction (2P-FOCUS). 2P-FOCUS corrects scattering by intensity modulation in the Fourier domain, leveraging the nonlinearity of multiple-beam interference and two-photon excitation, eliminating the need for a guide star, iterative optimization, or measuring transmission or reflection matrices. 2P-FOCUS uses random patterns to probe scattering properties, combined with a single-shot algorithm to rapidly generate the correctio

SUBMITTER: Zepeda D 

PROVIDER: S-EPMC11844625 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature