NAT10-mediated mRNA <i>N</i>⁴-acetylation is essential for the translational regulation during oocyte meiotic maturation in mice.

ABSTRACT: The precise translational regulation of maternal messenger RNAs (mRNAs) drives mammalian oocyte maturation. However, the function and mechanism of posttranscriptional chemical modifications, especially the newly identified N⁴-acetylcytidine (ac⁴C) modification catalyzed by N-acetyltransferase 10 (NAT10), are unknown. In this study, we developed a low-input ac⁴C sequencing technology, ac⁴C LACE-seq, and mapped 8241 ac⁴C peaks at the whole-transcriptome level using 50 mouse oocytes at the germinal vesicle stage. Oocyte-specific Nat10 knockout wiped out ac⁴C signals in oocytes and caused severe defects in meiotic maturation and female infertility. Mechanically, Nat10 deletion led to a failure of ac

SUBMITTER: Chen L 

PROVIDER: S-EPMC11844725 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature