Designed mosaic nanoparticles enhance cross-reactive immune responses in mice.

ABSTRACT: Nanoparticle vaccines displaying combinations of SARS-like betacoronavirus (sarbecovirus) receptor-binding domains (RBDs) could protect against SARS-CoV-2 variants and spillover of zoonotic sarbecoviruses into humans. Using a computational approach, we designed variants of SARS-CoV-2 RBDs and selected 7 natural sarbecovirus RBDs, each predicted to fold properly and abrogate antibody responses to variable epitopes. RBDs were attached to 60-mer nanoparticles to make immunogens displaying two (mosaic-2_COMs), five (mosaic-5_COM), or seven (mosaic-7_COM) different RBDs for comparisons with mosaic-8b, which elicited cross-reactive antibodies and protected animals from sarbecovirus challenges. Naive and COVID-19 pre-vaccinated mice immunized with mosaic-7_COM

SUBMITTER: Wang E 

PROVIDER: S-EPMC11845252 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature