Fast Processing of Electron Microscopic Specimen Preserved Ultrastructure of Glomeruli and Electron-Dense Deposits in Diagnostic Renal Biopsies: A Prospective and Retrospective Comparative Study.

ABSTRACT: Optimization of electron microscopy (EM) tissue processing protocols is essential to handle the global increase in the number of renal biopsies requiring EM for accurate diagnosis. The conventional EM processing method (CEM) is the standard method used by >95% of laboratories worldwide and it takes at least 48 to 52 hours for completion. In contrast, a fast-processing EM (FEM) method using microwave irradiation can be completed in 8 hours, allowing EM findings to be reported within 24 hours for most cases. There is widespread concern about the suboptimal quality of the FEM that may compromise its diagnostic roles; however, qualitative and quantitative data supporting the noninferiority of FEM compared with CEM has not been reported. We performed both prospective and retrospective studies.

SUBMITTER: Pinedo A 

PROVIDER: S-EPMC11845288 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature