ChIP provides 10-fold microbial DNA enrichment from tissue while minimizing bias.

ABSTRACT:

Background

Host DNA depletion is a critical tool for accessing the microbiomes of samples that have a small amount of microbial DNA contained in a high host background. Of critical practical importance is the ability to identify microbial DNA sequences in frozen tissue specimens. Here, we compare four existing commercial methods and two newly introduced methods involving chromatin immunoprecipitation (ChIP) on frozen human and pig intestinal biopsies.

Results

We find that all methods that rely on differential lysis of host and microbial cells introduce substantial biases as assessed by 16 S rRNA gene amplicon profiling. However, ChIP enables 10-fold enrichment of microbial DNA while introducing less bias, sufficient to make assessment possible against background, in both pig

SUBMITTER: Bhute S 

PROVIDER: S-EPMC11845529 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature