Aflatoxin B₁ (AFB₁) biodegradation by a lignolytic phenoloxidase of Trametes hirsuta.

ABSTRACT: Aflatoxin B₁ (AFB₁) is a highly potent mycotoxin that poses a serious threat to human and animal health. This study investigated the biodegradation of AFB₁ by the supernatant of submerged cultured Trametes hirsuta, with a focus on identifying and characterizing the responsible enzyme(s). The extracellular enzymes of the white-rot mushroom were extracted from the supernatant and pre-separated using anion exchange fast protein liquid chromatography (FPLC). To pinpoint the specific enzyme, the eluted protein fractions exhibiting the highest degradation activity were subjected to detailed biochemical and proteomic analyses. A second purification step, ultrafiltration, yielded an electrophoretically pure enzyme. Sequencing of tryptic peptides using a nano-LC sys

SUBMITTER: Soylemez T 

PROVIDER: S-EPMC11845786 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature