Reduction of enteric methane emission using methanotroph-based probiotics in Hanwoo steers.

ABSTRACT:

Background

Methane emission from enteric rumen fermentation is a main source of greenhouse gas (GHG) emission and a major concern for global warming.

Results

In this study, we isolated methanotroph-methylotroph consortium NC52PC from the rumen after a series of sub-culture and repetitive streaking on an agar plate and polycarbonate membrane filter. The NC52PC comprises methanotroph species (Methylocystis sp.) and methylotroph species (Methylobacterium sp.), forming a consortium capable of growing solely on methane as a carbon source. Their morphology, growth, and genome sequence were characterized. We assessed its effectiveness in mitigating methane emissions through both in vitro and in vivo experiments. During the in vitro trial, the introduction of NC52PC (at a concentrat

SUBMITTER: Tseten T 

PROVIDER: S-EPMC11846464 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature