Uncovering pre-cytokinetic block in cancer cells under shear stress using a disturbed flow-generating device.

ABSTRACT: During metastasis, cancer cells navigate through harsh conditions, including various mechanical forces in the bloodstream, highlighting the need to understand the impact of mechanical and shear stresses on cancer cells. To overcome the current methodological limitations of such research, here we present a new device that replicates similar conditions by applying shear stress on cultured cells. The device provides a less complex, easily accessible alternative to traditional fluidics while generating fluid shear stress values comparable to those in human veins and capillaries. The device allows analyses of large cell numbers in standard cell culture flasks and incubators. Using this device to explore the shear stress-induced responses of various human cell lines, we discovered a previously u

SUBMITTER: Beresova L 

PROVIDER: S-EPMC11846833 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature