Zbtb48 is a regulator of Mtfp1 expression in zebrafish.

ABSTRACT: ZBTB48 (also known as TZAP) is a transcription factor that has previously been reported to bind to telomeres and act as a negative regulator of telomere length in human cell lines. To explore whether transcription factor activity and telomere length regulation are conserved at the organismal level in vertebrates, we generate a zbtb48^-/- zebrafish line via CRISPR‒Cas genome editing. The zbtb48^-/- mutants display no obvious physical or behavioral abnormalities in the first two generations. We find no statistically significant changes in telomere length in first-generation adults. However, for the gene regulatory aspect of Zbtb48, similar to that in human cancer cell lines, we observe downregulation of mtfp1 at both the mRNA and protein levels in the zbtb48^-/-

SUBMITTER: Goh SYC 

PROVIDER: S-EPMC11846949 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature