ZHX2 inhibits diabetes-induced liver injury and ferroptosis by epigenetic silence of YTHDF2.

ABSTRACT:

Objective

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a common complication of type 2 diabetes mellitus (DM). The transcription factor zinc fingers and homeoboxes 2 (ZHX2) has been implicated in the pathogenesis of chronic liver diseases, yet its precise role and underlying mechanism in DM-induced hepatic injury remain poorly elucidated.

Methods

To investigate this, we used a high-fat diet (HFD) and streptozotocin (STZ) administration to create a DM model in mice, while high glucose (HG) exposure was used to simulate DM in vitro. Through various experiments such as luciferase reporter assay, chromatin immunoprecipitation, RNA immunoprecipitation, and rescue experiments, we aimed to uncover the mechanisms involving ZHX2.

Results

Our findings rev

SUBMITTER: Meng W 

PROVIDER: S-EPMC11846978 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature