Secretomes of Gingival Fibroblasts From Periodontally Diseased Tissues: A Proteomic Analysis.

ABSTRACT:

Objective

Cell secretomes represent a promising strategy for periodontal and bone regeneration. The objective of this study was to characterize the secretome of human gingival fibroblasts (GF) from periodontally diseased tissues (GF-perio) using proteomics.

Materials and methods

Conditioned media of GF-perio from periodontitis patients (n = 6, 48-h serum-free culture) were subjected to liquid chromatography with tandem mass spectrometry. Global profiles, differentially expressed proteins (DEPs), and functional/gene-set enrichment (FEA) were analyzed using bioinformatics. Selected bone regeneration-related proteins were additionally measured using a multiplex immunoassay. Conditioned media of GF from periodontally healthy subjects were used as a reference.

Results

Over

SUBMITTER: Smedas AK 

PROVIDER: S-EPMC11847645 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature