SHP2 is essential for the progesterone-promoted proliferation and migration in breast cancer cell lines.

ABSTRACT:

Introduction

We previously demonstrated that progesterone (P4) can promote breast cancer cell proliferation and migration through activating the P4 receptor (PR)/cSrc-mediated signaling pathway. It has been suggested that high level of Src homology region 2 domain-containing phosphatase-2 (SHP2) might be involved in breast oncogenesis. This study aimed to investigate whether SHP2 is involved in the P4-mediated cSrc activation in breast cancer cells.

Methods

T47D, MCF-7 and BT-483 breast cancer cell lines were used in this study. Cell proliferation and migration were examined using MTT technique and wound healing assay, respectively. Immunoprecipitation assay and Western blot analysis were performed to evaluate protein-protein interaction and protein expression, respectively.

SUBMITTER: Wang HC 

PROVIDER: S-EPMC11847685 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature