IPSC-derived exosomes as amphotericin B carriers: a promising approach to combat cryptococcal meningitis.

ABSTRACT:

Background

Cryptococcal meningitis (CM) is a significant global health issue, particularly affecting individuals with HIV. Amphotericin B (AmB) serves as the cornerstone treatment for CM; however, its clinical application is restricted due to limited penetration of the blood-brain barrier and associated nephrotoxicity.

Objective

This study investigates the use of exosomes derived from induced pluripotent stem cells (iPSC-Exos) as carriers for AmB in treating CM, aiming to enhance therapeutic efficacy and safety and reduce AmB toxicity.

Methods

Exosomes were extracted from iPSC culture supernatants using ultrafiltration and ultracentrifugation. Their morphology and size were analyzed using transmission electron microscopy (TEM) and nanoparticle flow cytometry (nFCM). P

SUBMITTER: Zhao J 

PROVIDER: S-EPMC11847882 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature