CD163, a novel receptor for TNF, was revealed in situ by proximity ligation assay.

ABSTRACT: Cytokine therapy utilizes cytokines to enhance the immune system to fight diseases. These strategies rely on advanced knowledge, including the communication between cytokines and their receptors. In situ, cytokine-receptor interactions are typically analyzed by co-localization using immunolabeling. Our study compared co-localization using the Proximity Ligation Assay (PLA), a recently developed in situ protein-protein interaction technique. In an inflamed porcine lung model, we demonstrated the efficacy of PLA in detecting interactions between tumor necrosis factor (TNF) and its receptors TNFR1 and TNFR2. Additionally, the CD163 receptor was identified as a novel partner of TNF. Furthermore, the combination of immunolabeling and PLA offered additional insights, particularly,

SUBMITTER: Cousin A 

PROVIDER: S-EPMC11848091 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature