Allosteric genetically encoded biosensor for spatiotemporal monitoring of endogenous RNA dynamics in living cells.

ABSTRACT: Functions of RNAs are associated with their abundance and unique subcellular localizations. RNA imaging methods for spatiotemporal monitoring of RNA dynamics would facilitate the discovery of unknown functions of RNA, yet improving RNA imaging is challenging because of limitations in methods for directly monitoring native RNA, especially the dynamics of RNA transport and concentration fluctuation. Herein, a label-free and conformation switching-based genetically encoded sensor, termed the Dual-locked RNAtracker (Ducker), that realizes spatiotemporal monitoring of endogenous RNA dynamics in living cells is developed. In this Ducker system, a distinctive strategy is developed by employing one RNA target to initiate an allosteric event that triggers the two locked fluorogenic RNA aptamer (M18

SUBMITTER: Yuan D 

PROVIDER: S-EPMC11848333 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature