Ratio-based indicators for cytosolic Ca²⁺ with visible light excitation.

ABSTRACT: Calcium ions (Ca²⁺) play central roles in cellular physiology. Fluorescent indicators for Ca²⁺ ions revolutionized our ability to make rapid, accurate, and highly parallel measurement of Ca²⁺ concentrations in living cells. The use of ratio-based imaging with one particular indicator, fura-2, allowed practitioners to correct for a number of experimental confounds, including dye bleaching, variations in sample thickness, and fluctuations in illumination intensity. Ratio-based imaging with fura-2 was the most accurate and reliable method for measuring Ca²⁺ concentrations. Two drawbacks to fura-2 exist. First, it requires ultraviolet (UV) excitation, which is more toxic to living cells than visible light. Second, our ability to use fura-2 for accura

SUBMITTER: Zhou X 

PROVIDER: S-EPMC11848355 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature