Benchmarking of a multi-biomarker low-volume panel for Alzheimer's disease and related dementia research.

ABSTRACT:

Introduction

In the research setting, obtaining accurate established biomarker measurements and maximizing use of the precious samples is key. Accurate technologies are available for Alzheimer's disease (AD), but no platform can measure all the established and emerging biomarkers in one run. The NUcleic acid Linked Immuno-Sandwich Assay (NULISA) is a technology that requires 15 µL of sample to measure more than 100 analytes.

Methods

We compared AD-relevant biomarkers included in the NULISA against validated assays in cerebrospinal fluid (CSF) and plasma.

Results

CSF measures of amyloid beta 42/40, and phosphorylated tau (p-tau)217 are highly correlated when measured by immunoassay, mass spectrometry, or NULISA. In plasma, p-tau217 performance is similar to that report

SUBMITTER: Ibanez L 

PROVIDER: S-EPMC11848383 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature