Photopic flicker optoretinography captures the light-driven length modulation of photoreceptors during phototransduction.

ABSTRACT: In this study, we used an inhibitor of phosphodiesterase 6 (PDE6) to examine the impact of changes in the conformation of the PDE6 protein on the light-induced process responsible for altering the length of the outer segments of photoreceptor cells in both human and rodent eyes. We employed a imaging method called spatiotemporal optical coherence tomography, which ensures high contrast and phase stability within the strongly scattering photoreceptor- Retinal Pigment Epithelium complex. Using this approach, we recorded nanometer-scale changes in human cones and rods in response to photopic flicker stimulation and observed length changes in rodent rods under scotopic conditions following a single pulse of light, in the absence or presence of sildenafil, which inhibits the catalytic activity

SUBMITTER: Tomczewski S 

PROVIDER: S-EPMC11848411 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature