High protein diet increases the risk of allergic sensitization but not asthma in mice through modulation of the cytokine milieu toward Th2 bias.

ABSTRACT:

Introduction

The role of different nutrients in allergic sensitization is not clear. In this study we aimed to determine the effect of high protein (HP) diet on allergic sensitization, cytokine profile, and asthma in mice.

Methods

Seven- to eight-week old female BALB/c mice were fed either normal (ND) or HP diet and were sensitized with ovalbumin intraperitoneally followed by intranasal challenge. Allergic sensitization was tested by measuring anti-ovalbumin (OVA) IgE, IgG1, and IgG2a antibodies. Cytokine levels were tested by multiplex ELISA in splenocyte supernatants after stimulation. Airway inflammation was tested by measuring total and differential cell counts in bronchoalveolar lavage fluid and by measuring bronchial mucus production, goblet cell hyperplasia and periva

SUBMITTER: Al-Herz W 

PROVIDER: S-EPMC11848459 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature