Characterization of subcutaneous and visceral de-differentiated fat cells.

ABSTRACT:

Objective

The capacity of mature adipocytes to de-differentiate into fibroblast-like cells has been demonstrated in vitro and a few, rather specific in vivo conditions. A detailed comparison between de-differentiated fat (DFAT) cells and adipose stem and progenitor cells (ASPCs) from different adipose depots is yet to be conducted. Moreover, whether de-differentiation of mature adipocytes from classical subcutaneous and visceral depots occurs under physiological conditions remains unknown.

Methods

Here, we used in vitro "ceiling culture", single cell/nucleus RNA sequencing, epigenetic anaysis and genetic lineage tracing to address these unknowns.

Results

We show that in vitro-derived DFAT cells have lower adipogenic potential and distinct cellular composition compared

SUBMITTER: Li Y 

PROVIDER: S-EPMC11848481 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature