Survival and Developmental Progression of Unselected Thymocytes in the Absence of the T-Cell Adaptor Gads.

ABSTRACT: Thymocyte β-selection and positive-selection depend on TCR signaling via the immune adaptors SLP-76 and LAT. Gads bridges the recruitment of SLP-76 to LAT, yet is not required for the maturation of single positive (SP) thymocytes. To illuminate this paradox, we performed tamoxifen-induced ablation of Gads (Gads^iKO), accompanied by the expression of tdTomato, and compared the development of Gads-expressing (Tom^-) and Gads-ablated (Tom⁺) thymocytes within the same mouse. Gads^iKO (Tom⁺) thymocytes exhibited impaired β- and positive-selection, yet δ-selection was not affected. While susceptible to apoptosis ex vivo, the marked accumulation of self-MHC nonresponding (CD5^-) Gads^iKO DP thymocytes suggested the possibilit

SUBMITTER: Shalah R 

PROVIDER: S-EPMC11848708 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature