Four Target Resequencing for the Bovine Major Histocompatibility Complex Region. Proof of Concept.

ABSTRACT: The bovine leukocyte antigen (BoLA) comprises four regions that contain a high density of polymorphic genes and frequently show gene copy number variations (CNV). Therefore, genotyping BoLA using genome-wide resequencing is difficult. This study aimed to develop four probe sets for resequencing of the BoLA region using a hybridization capture target next-generation sequencing (NGS) method. This proof of concept showed and discussed the several applications of the used strategy. DNAs from nine Japanese Black cows and one Holstein cow were genotyped for BoLA-DRB3 using PCR sequence-based typing (SBT). DNA libraries were constructed using the KAPA HyperPlus Kit, and BoLA DNA sequences were enriched using the SeqCap EZ kit and four custom-made probes. Based on preliminary results, the probe se

SUBMITTER: Giovambattista G 

PROVIDER: S-EPMC11848996 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature