OCT4 translationally promotes AKT signaling as an RNA-binding protein in stressed pluripotent stem cells.

ABSTRACT:

Background

Despite numerous studies addressing the molecular mechanisms by which pluripotent stem cells (PSCs) maintain self-renewal and pluripotency under normal culture conditions, the fundamental question of how PSCs manage to survive stressful conditions remains largely unresolved. Post-transcriptional/translational regulation emerges to be vital for PSCs, but how PSCs coordinate and balance their survival and differentiation at translational level under extrinsic and intrinsic stress conditions is unclear.

Methods

The high-throughput sequencing of cross-linking immunoprecipitation cDNA library (HITS-CLIP) was employed to decipher the genome-wide OCT4-RNA interactome in human PSCs, a combined RNC-seq/RNA-seq analysis to assess the role of OCT4 in translational regulation

SUBMITTER: Chen W 

PROVIDER: S-EPMC11849194 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature