A digital microfluidic approach to increasing sample volume and reducing bead numbers in single molecule array assays.

ABSTRACT: We report methods that improve the manipulation of magnetic beads using digital microfluidics (DMF) that can enhance the performance of single molecule array (Simoa) digital protein assays in miniaturized analytical systems. Despite significant clinical and biomedical applications for digital protein detection, the development of miniaturized Simoa systems has been limited by the requirements for use of large sample volumes (∼100 μL) and low numbers of beads (∼5000) for high sensitivity tests. To address these challenges, we improved the integration of DMF with Simoa-based assays by developing strategies for loading mixtures of sample and beads into DMF networks using methods relying on either virtual channels or small liquid segments that were applied either in parallel or in a stepwise m

SUBMITTER: Salari A 

PROVIDER: S-EPMC11849296 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature