Clinical application of targeted nanopore sequencing in pathogen detection in patients with sepsis.

ABSTRACT:

Background

Precision in detecting pathogens in sepsis patients is crucial for deploying targeted therapeutic strategies. The objective of the present investigation was to assess the efficacy of targeted nanopore sequencing (TNPseq) as a method for detecting sepsis pathogens.

Methods

For this investigation, we stratified 90 patients with sepsis into 'improved' and 'unimproved' groups based on their clinical outcomes. Pathogenic microorganisms were detected in various sample types on the day of sepsis diagnosis, utilizing both conventional culture and TNPseq techniques. A comparative analysis of these methodologies was undertaken to assess their performance and efficacy in identifying pathogens in sepsis infections and discern pathogens with significant differences between the

SUBMITTER: Chen M 

PROVIDER: S-EPMC11849374 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature