Metagenomic immunoglobulin sequencing reveals IgA coating of microbial strains in the healthy human gut.

ABSTRACT: IgA, the primary human antibody secreted from the gut mucosa, shapes the intestinal microbiota. Methodological limitations have hindered defining which microbial strains are targeted by IgA and the implications of binding. Here we develop a technique, metagenomic immunoglobulin sequencing (MIg-seq), that provides strain-level resolution of microbes coated by IgA and use it to determine IgA coating levels for 3,520 gut microbiome strains in healthy human faeces. We find that both health and disease-associated bacteria are targeted by IgA. Microbial genes are highly predictive of IgA binding levels; in particular, mucus degradation genes are correlated with high binding, and replication rates are significantly reduced for microbes bound by IgA. We demonstrate that IgA binding is more correla

SUBMITTER: Olm MR 

PROVIDER: S-EPMC11849979 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature