Bioprinting of mesenchymal stem cells in low concentration gelatin methacryloyl/alginate blends without ionic crosslinking of alginate.

ABSTRACT: Bioprinting allows for the fabrication of tissue-like constructs by precise architecture and positioning of the bioactive hydrogels with living cells. This study was performed to determine the effect of very low concentrations of alginate (0.1, 0.3, and 0.5% w/v) on bioprinting of bone marrow mesenchymal stem cells (BMSC) in gelatin methacryloyl (GelMA; 5% w/v)/alginate blend. Furthermore, while GelMA was photocrosslinked in all bioprinted constructs, the effect of crosslinking alginate with calcium chloride on the physical and biological characteristics of the constructs was investigated. The inclusion of low-concentration alginate improved the viscosity and printability of the formulation as well as the compressive modulus of the hydrogels, particularly when ionically crosslinked with ca

SUBMITTER: Jahani Kadousaraei M 

PROVIDER: S-EPMC11850620 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature