Knockdown of LncRNA MEG3 promotes damage of vascular endothelial cells induced by vibration.

ABSTRACT: Hand-arm vibration syndrome (HAVS) is caused by long-term exposure to hand-transmitted vibration (HTV), and its pathogenesis has not been elucidated fully. We explored the molecular mechanism of HAVS and provided clues and a theoretical basis for the early prevention and treatment of HAVS. After vibration, samples were collected from the plasma of human workers, plasma of rat tails, and human umbilical vein endothelial cells (HUVECs). ELISAs were used to measure the expression of vasoactive factors. Cell Counting Kit-8 and electron microscopy were used to detect cell damage. Flow cytometry was employed to detect apoptosis. Real-time reverse transcription-polymerase chain reaction was used to measure the expression of long non-coding RNAs (lncRNAs). Western blotting was used to measure the

SUBMITTER: Hu X 

PROVIDER: S-EPMC11850629 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature