Comparisons of aged samples and modern references provide algorithm for mtDNA analysis in challenging material.

ABSTRACT: Based on results of over 12 years of research, we performed a comparative analysis of haplotypes from 70 to 80 years old bone samples with modern high quality references. Whole mitochondrial genomes were obtained for the purpose of human identification cases conducted by the Polish Genetic Database of Victims of Totalitarianisms-Pomeranian Medical University Research Centre, using Thermo Fisher Scientific's Precision ID line and Ion GeneStudio S5. Converge 2.2 and IGV 2.12.3 were used for secondary sequence analysis and their parameters were altered to construct a new variant calling algorithm. We have found neither a simple change in thresholds, nor removing contaminant reads significantly decreased the number of discrepancies found between haplotype pairs, and conclude that standard anal

SUBMITTER: Szargut M 

PROVIDER: S-EPMC11850911 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature