Dissecting the properties of circulating IgG against streptococcal pathogens through a combined systems antigenomics-serology workflow.

ABSTRACT: This study showcases an integrative mass spectrometry-based strategy combining systems antigenomics and systems serology to characterize human antibodies in clinical samples. This strategy involves using antibodies circulating in plasma to affinity-enrich antigenic proteins in biochemically fractionated pools of bacterial proteins, followed by their identification and quantification using mass spectrometry. A selected subset of the identified antigens is then expressed recombinantly to isolate antigen-specific IgG, followed by characterization of the structural and functional properties of these antibodies. We focused on Group A streptococcus (GAS), a major human pathogen lacking an approved vaccine. The data shows that both healthy and GAS-infected individuals have circulating IgG against

SUBMITTER: Gomez Toledo A 

PROVIDER: S-EPMC11850916 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature