Mechanistic studies of Ca²⁺-induced classical pyroptosis pathway promoting renal adhesion on calcium oxalate kidney stone formation.

ABSTRACT: This study aims to investigate the role of hypercalciuria and pyroptosis in the formation of calcium oxalate kidney stones. Bioinformatics analysis was performed to compare the correlation of pyroptosis scores and cell adhesion scores between Randall's plaques and normal tissues from kidney stone patients. For the in vitro experiments, we investigated the effects of high concentrations of Ca²⁺ on the pyroptosis and adhesion levels of renal tubular epithelial cells and examined the adhesion levels and crystal aggregation of the cells in high Ca²⁺ concentrations environment by knockdown and overexpression of the key pyroptosis gene, GSDMD, and we verified the effects of Ca²⁺ concentration on pyroptosis and adhesion levels, kidney injury, and crystal depositio

SUBMITTER: Xiang J 

PROVIDER: S-EPMC11850917 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature