DNA replication dynamics are associated with genome composition in Plasmodium species.

ABSTRACT: Plasmodium species have variable genome compositions: many have an A/T content >80%, while others are similar in composition to human cells. Here, we made a direct comparison of DNA replication dynamics in two Plasmodium species whose genomes differ by ∼20% A/T content. This yielded fundamental insights into how DNA composition may affect replication. The highly A/T-biased genome of Plasmodium falciparum showed unusual replication dynamics that were not observed in the more balanced Plasmodium knowlesi-which had dynamics more like those of human cell lines. Replication forks moved 50% slower in P. falciparum than in P. knowlesi. In P. falciparum, replication forks slowed down over the course of S-phase, whereas in P. knowlesi, fork speed increased as in human cells. Furthermore, in both P.

SUBMITTER: Totanes FIG 

PROVIDER: S-EPMC11851108 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature