Hydroperoxide-Independent Generation of Spin Trapping Artifacts by Quinones and DMPO: Implications for Radical Identification in Quinone-Related Reactions.

ABSTRACT: Quinones, as highly redox active molecules in biology, are believed to react with hydroperoxides to produce highly reactive ^•OH, assuming that radical adducts are exclusively formed by the addition of free radicals to the spin trap as detected by the electron paramagnetic resonance (EPR) methodology. Here, direct formation of the same DMPO adduct as that formed by genuine radical trapping of ^•OH is discovered, while quinones (i.e., 1,4-benzoquinone (BQ), methyl-BQ (2-Me-BQ, 2,5-Me-BQ, 2,6-Me-BQ), and chlorinated-BQ (2-Cl-BQ, 2,5-Cl-BQ, 2,6-Cl-BQ)) meet with the spin trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO), independent of peroxides. According to differences in alcohol-derived adducts (e.g., DMPO-CH₂OH or DMPO-OCH₃) while alcohol is att

SUBMITTER: Wang L 

PROVIDER: S-EPMC11851217 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature