A High Throughput Microplate Feeder Assay for Quantification of Consumption in Drosophila.

ABSTRACT: Quantifying food intake in Drosophila is used to study the genetic and physiological underpinnings of consumption-associated traits, their environmental factors, and the toxicological and pharmacological effects of numerous substances. Few methods currently implemented are amenable to high throughput measurement. The Microplate Feeder Assay (MFA) was developed for quantifying the consumption of liquid food for individual flies using absorbance. In this assay, flies consume liquid food medium from select wells of a 1536-well microplate. By incorporating a dilute tracer dye into the liquid food medium and loading a known volume into each well, absorbance measurements of the well acquired before and after consumption reflect the resulting change in volume (i.e., volume consumed). To enable hi

SUBMITTER: Walters JD 

PROVIDER: S-EPMC11851349 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature